Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 5 November 1971:
Vol. 174. no. 4009, pp. 593 - 598
DOI: 10.1126/science.174.4009.593
Prev | Table of Contents | Next

Articles

Interferon Action: Inhibition of Vesicular Stomatitis Virus RNA Synthesis Induced by Virion-Bound Polymerase

Philip I. Marcus 1, Dean L. Engelhardt 1, John M. Hunt 1, and Margaret J. Sekellick 1

1 University of Connecticut, Storrs 06268

The particle-bound RNA polymerase activity of vesicular stomatitis virus (VSV) can be demonstrated in vivo. Linear synthesis of viral RNA persists for 5 to 6 hours at 34°C in infected monolayers of chick embryo cells treated with cycloheximide and actinomycin D to block synthesis of protein and cell-specific RNA. At least 55 percent of the RNA made under these conditions is complementary to virion RNA. RNA synthesis mediated by VSV polymerase activity is inhibited in cells first treated with chick-derived interferon or polyriboinosinate• polyribocytidylate, but not by mouse interferon. The RNA product of VSV polymerase activity is present throughout the cytoplasm, and its synthesis is inhibited by the interferon system, as judged by autoradiographs that show the physical distribution, in cells, of RNA produced by virion polymerase in the absence of translation—a demonstration of the transcription product of the viral genome.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Herpes Simplex Virus ICP0 Mutants Are Hypersensitive to Interferon.
K. L. Mossman, H. A. Saffran, and J. R. Smiley (2000)
J. Virol. 74, 2052-2056
   Abstract »    Full Text »
Possible Involvement of the Double-Stranded RNA-Binding Core Protein sigma A in the Resistance of Avian Reovirus to Interferon.
J. Martínez-Costas, C. González-López, V. N. Vakharia, and J. Benavente (2000)
J. Virol. 74, 1124-1131
   Abstract »    Full Text »
Selective inhibition of glycoprotein and membrane protein of vesicular stomatitis virus from interferon-treated cells.
R. Maheshwari, F. Jay, and R. Friedman (1980)
Science 207, 540-541
   Abstract »    PDF »
Progress in Virology: Pathways in the Progression of Respiratory Tract Invasions.
J. W. St. Geme JR (1976)
Clinical Pediatrics 15, 164-168
   PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)