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Science 29 October 1971:
Vol. 174. no. 4008, pp. 507 - 510
DOI: 10.1126/science.174.4008.507
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Articles

Polyadenylic Acid Sequences: Role in Conversion of Nuclear RNA into Messenger RNA

J. E. Darnell 1, L. Philipson 1, R. Wall 1, and M. Adesnik 1

1 Department of Biological Sciences, Columbia University, New York 10027

Polyadenylic acid [poly(A)] segments containing 150 to 250 nucleotides appear to be covalently linked to heterogeneous nuclear RNA (HnRNA) and messenger RNA (mRNA) in eucaryotic cells. The poly(A) is synthesized in the nucleus, and is probably linked initially to HnRNA that is ultimately transported as mRNA to the cytoplasm. Studies with inhibitors of RNA or poly(A) synthesis indicate that synthesis of poly(A) segments is independent of transcription. The poly(A) marker may prove useful to elucidate mRNA modification and transport in eucaryotic cells.

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