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Science 22 October 1971:
Vol. 174. no. 4007, pp. 412 - 415
DOI: 10.1126/science.174.4007.412
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Articles

Freezing and Melting of Lipid Bilayers and the Mode of Action of Nonactin, Valinomycin, and Gramicidin

Sally Krasne 1, George Eisenman 1, and Gabor Szabo 1

1 Department of Physiology, University of California School of Medicine, Los Angeles 90024

An abrupt loss of effectiveness of the presumed carriers, nonactin and valinomycin, in mediating ion conductance occurred at the same temperature as the membrane fluidity, judged visually, was lost. By contrast, the effects of the presumed channel-former, gramicidin, were the same on solid and liquid membranes. Taken together, these findings imply that freezing the membrane primarily reduces the mobility of these antibiotics with little effect on their solubility.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)