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Science 17 September 1971:
Vol. 173. no. 4002, pp. 1142 - 1143
DOI: 10.1126/science.173.4002.1142
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Articles

Cyclic Adenosine Monophosphate in Brain Areas: Microwave Irradiation as a Means of Tissue Fixation

Michale J. Schmidt 1, Dennis E. Schmidt 1, and G. Alan Robison 1

1 Tennessee Neuropsychiatric Institute and Department of Pharmacology, Vanderbilt University School of Medicine, Nashville 37217

Amounts of cyclic adenosine monophosphate in discrete regions of the brain were estimated after exposure of rats to microwave irradiation. Amounts were highest in the cerebellum and brainstem, intermediate in the hypothalamus and midbrain, and lowest in the hippocampus and cortex. Decapitation increased the concentration of cyclic adenosine monophosphate in all brain areas, although the increase in the cerebellum was three to four times greater than that in other areas. Microwave irradiation may provide a means of rapidly fixing brain tissue in situ while permitting easy dissection of the brain. In this way artifacts produced by decapitation can be eliminated, and concentrations of heat-stable compounds in the brain can be estimated under conditions which more closely approximate those in vivo.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cyclic Adenosine Monophosphate in Cerebral Cortex: Alterations Following Trauma.
H. Watanabe and J. V. Passonneau (1975)
Arch Neurol 32, 181-184
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Cyclic Adenosine Monophosphate, Metabolites, and Phosphorylase in Neural Tissue: A Comparison of Methods of Fixation.
W. D. Lust, J. V. Passonneau, and R. L. Veech (1973)
Science 181, 280-282
   Abstract »    PDF »
Cyclic Adenosine Monophosphate and Hypertension in Rats.
M. S. Amer (1973)
Science 179, 807-809
   Abstract »    PDF »
Adenosine 3',5'-Monophosphate Is Localized in Cerebellar Neurons: Immunofluorescence Evidence.
F. E. Bloom, B. J. Hoffer, E. R. Battenberg, G. R. Siggins, A. L. Steiner, C. W. Parker, and H. J. Wedner (1972)
Science 177, 436-438
   Abstract »    PDF »


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