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Science 2 July 1971:
Vol. 173. no. 3991, pp. 51 - 54
DOI: 10.1126/science.173.3991.51
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Articles

1,25-Dihydroxycholecalciferol: Identification of the Proposed Active Form of Vitamin D3 in the Intestine

Anthony W. Norman 1, James F. Myrtle 1, Ronald J. Miogett 1, Henry G. Nowicki 1, Vincent Williams 2, and G. Popjaák 2

1 Department of Biochemistry, University of California, Riverside 92502
2 Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024

The major polar metabolite of cholecalciferol (vitamin D3) present in chick intestinal mucosa has been chemically characterized by mass spectrometric analysis to have a molecular formula of C27H4403 and a structure of 1,25-dihydroxycholecalciferol. This compound, which is produced in the kidney from 25-hydroxycholecalciferol, has been previously shown to be from 4 to 13 times as active as cholecalciferol in stimulating intestinal calcium transport. 1,25-Dihydroxycholecalciferol (previously designated metabolite 4B in this (laboratory) probably represents the biologically active form of cholecalciferol in the intestine.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Vitamin D Receptor: New Assignments for an Already Busy Receptor.
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Structure-Function Analysis of Vitamin D 24-Hydroxylase (CYP24A1) by Site-Directed Mutagenesis: Amino Acid Residues Responsible for Species-Based Difference of CYP24A1 between Humans and Rats.
H. Hamamoto, T. Kusudo, N. Urushino, H. Masuno, K. Yamamoto, S. Yamada, M. Kamakura, M. Ohta, K. Inouye, and T. Sakaki (2006)
Mol. Pharmacol. 70, 120-128
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Commentary: Sunlight, vitamin D, and the cancer connection revisited.
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Characterization of recombinant CYP2C11: a vitamin D 25-hydroxylase and 24-hydroxylase.
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Targeted ablation of the 25-hydroxyvitamin D 1alpha -hydroxylase enzyme: Evidence for skeletal, reproductive, and immune dysfunction.
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PNAS 98, 7498-7503
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Differential Use of Transcription Activation Function 2 Domain of the Vitamin D Receptor by 1,25-Dihydroxyvitamin D3 and Its A Ring-Modified Analogs.
S. Peleg, C. Nguyen, B. T. Woodard, J.-K. Lee, and G. H. Posner (1998)
Mol. Endocrinol. 12, 525-535
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Calcinogenic factor in Solanum malacoxylon: evidence that it is 1,25-dihydroxyvitamin D3-glycoside.
R. Wasserman, J. Henion, M. Haussler, and T. McCain (1976)
Science 194, 853-855
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Regulation of serum 1alpha,25-dihydroxyvitamin D3 by calcium and phosphate in the rat.
M. Hughes, P. Brumbaugh, M. Hussler, J. Wergedal, and D. Baylink (1975)
Science 190, 578-580
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Pharmacology and Therapeutics of Bone.
W.C. Sturtridge (1975)
Journal of Dental Research 54, B78-B86
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Radioreceptor Assay for 1agr,25-Dihydroxyvitamin D3.
P. F. Brumbaugh, D. H. Haussler, R. Bressler, and M. R. Haussler (1974)
Science 183, 1089-1091
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Intestinal Absorption of Calcium.
L. V. Avioli (1972)
Arch Intern Med 129, 345-355
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