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Science 11 June 1971:
Vol. 172. no. 3988, pp. 1134 - 1136
DOI: 10.1126/science.172.3988.1134
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Articles

Salt Extraction of Soluble HL-A Antigens

R. A. Reisfeld 1, M. A. Pellegrino 1, and B. D. Kahan 2

1 Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
2 Department of Surgery, Massachusetts General Hospital, Boston 02114

Extraction of cultured human lymphoid cells with hypertonic salt solutions (3 molar potassium chloride) resulted in high recoveries of membrane-associated histocompatibility (HL-A) antigens in soluble form with potent activity and marked immunologic specificity. The active principle was purified by preparative acrylamide-gel electrophoresis. Application of the hypertonic salt extraction method is now yielding sufficient HL-A antigen to begin the elucidation of the molecular basis of transplantation individuality.


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