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Science 30 April 1971:
Vol. 172. no. 3982, pp. 472 - 474
DOI: 10.1126/science.172.3982.472
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Articles

Cell Surface Antigens: Serial Sectioning of Single Cells as an Approach to Topographical Analysis

Christopher W. Stackpole 1, Tadao Aoki 1, Edward A. Boyse 1, Lloyd J. Old 1, Joseph Lumley-Frank 1, and Etienne de Harven 1

1 Sloan-Kettering Institute for Cancer Research, New York 10021

The topographical distribution of H-2 antigens on the surfaces of C57BL/6 mouse thymocytes and lymph-node lymphocytes was investigated by a new technique. Single cells were reacted with visually labeled antibody directed against H-2 antigen. Each cell was processed for electron microscopy individually and was serially sectioned. Models constructed from serial electron micrographs provided detailed views of the entire cell surface and showed that H-2 antigen occurs on thymocytes in small isolated regions and on lymphocytes in large interconnected regions.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
H-2 Antigen on Cell Membranes: An Explanation for the Alteration of Distribution by Indirect Labeling Techniques.
W. C. Davis (1972)
Science 175, 1006-1008
   Abstract »    PDF »
Golgi Apparatus: Influence on Cell Surfaces.
W. G. Whaley, M. Dauwalder, and J. E. Kephart (1972)
Science 175, 596-599
   PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)