Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 23 April 1971:
Vol. 172. no. 3981, pp. 384 - 385
DOI: 10.1126/science.172.3981.384
Prev | Table of Contents | Next

Articles

Preparation of Highly Labeled [32P]Nucleic Acids from Yeast; Isolation of "Denaturable" Leucine Acceptor Transfer RNA

Steven Kowalski 1 and Jacques R. Fresco 1

1 Department of Biochemical Sciences, Frick Chemical Laboratory, Princeton University, Princeton, New Jersey 08540

Culture conditions were developed that permit efficient incorporation of [32P]phosphate into nucleic acids of baker's yeast. RNA sufficiently labeled for sequence determination was obtained in this way. Pure "denaturable" leucine acceptor transfer RNA (tRNALeu3) was isolated for this purpose.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Nucleotide Sequence of the "Denaturable" Leucine Transfer RNA from Yeast.
S. Kowalski, T. Yamane, and J. R. Fresco (1971)
Science 172, 385-387
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)