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Science 9 April 1971:
Vol. 172. no. 3979, pp. 167 - 169
DOI: 10.1126/science.172.3979.167
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Articles

Microsomal Hydroxylase Induction in Liver Cell Culture by Phenobarbital, Polycyclic Hydrocarbons, and p,p'-DDT

J. E. Gielen 1 and D. W. Nebert 1

1 Section on Developmental Enzymology, Laboratory of Biomedical Sciences, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20014

Aryl hydrocarbon hydroxylase induction by phenobarbital, polycyclic hydrocarbons, and the insecticide, 2.2-bis(p-chlorophenyl)-1,1,1-trichloroethane (p,p'-DDT), occurs in rat fetal liver cell cultures. The maximum net rate at which the hydroxylase activity accumulates is about the same when phenobarbital, 3-methlcholanthrene, or benz[a]anthracene is in the growth medium at optimum concentrations. An additive effect is obtained when either phenobarbital or p.p'-DDT is present with a polycyclic hydrocarbon in the growth medium, but not when the cells are treated with phenobarbital plus p.p'-DDT or with the combination of two polycyclic hydrocarbons.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cuticle Deposition in Imaginal Disks: Effects of Juvenile Hormone and Fat Body in vitro.
H. Oberlander and C. Tomblin (1972)
Science 177, 441-442
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Induction of Benzo[agr]pyrene Hydroxylase in Human Skin.
W. Levin, A. H. Conney, A. P. Alvares, I. Merkatz, and A. Kappas (1972)
Science 176, 419-420
   Abstract »    PDF »


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