Cell-Free Protein Synthesizing System from Yeast Mitochondria
Alan H. Scragg 1,
Hideo Morimoto 1,
Vincente Villa 1,
John Nekhorocheff 1, and
Harlyn O. Halvorson 1
1 Laboratory of Molecular Biology, University of Wisconsin, 1525 Linden Drive, Madison 53706
An active, cell-free protein synthesizing system has been obtained from yeast mitochondria. The system is stimulated by both polyuridylate and R17 RNA and is sensitive to inhibitors of bacterial protein synthesizing systems. A comparison is made between this system and that found in the cytoplasm of yeast.