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Science 6 November 1970:
Vol. 170. no. 3958, pp. 633 - 635
DOI: 10.1126/science.170.3958.633
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Articles

Radioreceptor Assay of Adrenocorticotropic Hormone: New Approach to Assay of Polypeptide Hormones in Plasma

R. J. Lefkowitz 1, J. Roth 1, and I. Pastan 2

1 National Institute of Arthritis and Metabolic Diseases, Bethesda, Maryland 20014
2 National Cancer Institute, Bethesda, Maryland 20014

Biologically active iodine-125-labeled adrenocorticotropic hormone (ACTH) binds specifically to ACTH receptors extracted from adrenals. Unlabeled ACTH at 1 picogram per milliliter significantly displaces labeled ACTH from these receptors. This system, which appears to be applicable to all polypeptide hormones, provides a rapid and sensitive method for measurements of biologically active ACTH in dilute whole plasma.


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Radioreceptor Assay of Human Chorionic Gonadotropin: Detection of Early Pregnancy.
B. B. Saxena, S. H. Hasan, F. Haour, and M. Schmidt-Gollwitzer (1974)
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"Big" Growth Hormone Components from Human Plasma: Decreased Reactivity Demonstrated by Radioreceptor Assay.
P. Gorden, M. A. Lesniak, C. M. Hendricks, and J. Roth (1973)
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Radioreceptor Assay for Prolactin and Other Lactogenic Hormones.
R. P. C. Shiu, P. A. Kelly, and H. G. Friesen (1973)
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