Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 23 October 1970:
Vol. 170. no. 3956, pp. 447 - 449
DOI: 10.1126/science.170.3956.447
Prev | Table of Contents | Next

Articles

Specific Inhibition of Nuclear RNA Polymerase II by agr-Amanitin

Thomas J. Lindell 1, Fanyela Weinberg 1, Paul W. Morris 1, Robert G. Roeder 1, and William J. Rutter 1

1 Department of Biochemistry and Biophysics, University of California, San Francisco 94112

agr-Amanitin, a toxic substance from the mushroom Amanita phalloides, is a potent inhibitor of DNA-dependent RNA polymerase II (the nucleoplasmic form) from sea urchin, rat liver, and calf thymus. This compound exerts no effect on the activity of polymerase I (nucleolar form) or polymerase III (also nucleoplasmic). The inhibition is due to a specific interaction with polymerase II or with a complex of DNA and polymerase II.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Transcriptomic and proteomic analysis of global ischemia and cardioprotection in the rabbit heart.
J. D. McCully, M. K. Bhasin, C. Daly, M. C. Guerrero, S. Dillon, T. A. Liberman, D. B. Cowan, J. D. Mably, F. X. McGowan, and S. Levitsky (2009)
Physiol Genomics 38, 125-137
   Abstract »    Full Text »    PDF »
Patterns of Gene-Specific and Total Transcriptional Activity during the Plasmodium falciparum Intraerythrocytic Developmental Cycle.
J. S. Sims, K. T. Militello, P. A. Sims, V. P. Patel, J. M. Kasper, and D. F. Wirth (2009)
Eukaryot. Cell 8, 327-338
   Abstract »    Full Text »    PDF »
RNA Polymerase IV Functions in Paramutation in Zea mays.
K. F. Erhard Jr., J. L. Stonaker, S. E. Parkinson, J. P. Lim, C. J. Hale, and J. B. Hollick (2009)
Science 323, 1201-1205
   Abstract »    Full Text »    PDF »
Chromatin structure analyses identify miRNA promoters.
F. Ozsolak, L. L. Poling, Z. Wang, H. Liu, X. S. Liu, R. G. Roeder, X. Zhang, J. S. Song, and D. E. Fisher (2008)
Genes & Dev. 22, 3172-3183
   Abstract »    Full Text »    PDF »
Isolation of a Drosophila amplification origin developmentally activated by transcription.
F. Xie and T. L. Orr-Weaver (2008)
PNAS 105, 9651-9656
   Abstract »    Full Text »    PDF »
Histone Deacetylase Inhibition-Mediated Differentiation of RGC-5 Cells and Interaction with Survival.
B. R. Schwechter, L. E. Millet, and L. A. Levin (2007)
Invest. Ophthalmol. Vis. Sci. 48, 2845-2857
   Abstract »    Full Text »    PDF »
Genes associated with opening and senescence of Mirabilis jalapa flowers.
X. Xu, T. Gookin, C.-Z. Jiang, and M. Reid (2007)
J. Exp. Bot. 58, 2193-2201
   Abstract »    Full Text »    PDF »
Diagnosis of Amanita Toxicosis in a Dog with Acute Hepatic Necrosis.
B. Puschner, H. H. Rose, and M. S. Filigenzi (2007)
J Vet Diagn Invest 19, 312-317
   Abstract »    Full Text »    PDF »
Chromatin Structure and Transcription of the R1- and R2-Inserted rRNA Genes of Drosophila melanogaster.
J. Ye and T. H. Eickbush (2006)
Mol. Cell. Biol. 26, 8781-8790
   Abstract »    Full Text »    PDF »
Cytoplasmic IRE1{alpha}-mediated XBP1 mRNA Splicing in the Absence of Nuclear Processing and Endoplasmic Reticulum Stress.
S. H. Back, K. Lee, E. Vink, and R. J. Kaufman (2006)
J. Biol. Chem. 281, 18691-18706
   Abstract »    Full Text »    PDF »
Nuclear transcription is essential for specification of mammalian replication origins..
D. S. Dimitrova (2006)
Genes Cells 11, 829-844
   Abstract »    Full Text »    PDF »
Molecular Characterization and Inhibition of Amanitin Uptake into Human Hepatocytes.
K. Letschert, H. Faulstich, D. Keller, and D. Keppler (2006)
Toxicol. Sci. 91, 140-149
   Abstract »    Full Text »    PDF »
Structural Perspective on Mutations Affecting the Function of Multisubunit RNA Polymerases.
V. Trinh, M.-F. Langelier, J. Archambault, and B. Coulombe (2006)
Microbiol. Mol. Biol. Rev. 70, 12-36
   Abstract »    Full Text »    PDF »
Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells: nuclear mRNA export is coupled to ongoing gene transcription.
K. Tokunaga, T. Shibuya, Y. Ishihama, H. Tadakuma, M. Ide, M. Yoshida, T. Funatsu, Y. Ohshima, and T. Tani (2006)
Genes Cells 11, 305-317
   Abstract »    Full Text »    PDF »
RNA polymerase II synthesizes antisense RNA in Plasmodium falciparum.
K. T. MILITELLO, V. PATEL, A.-D. CHESSLER, J. K. FISHER, J. M. KASPER, A. GUNASEKERA, and D. F. WIRTH (2005)
RNA 11, 365-370
   Abstract »    Full Text »    PDF »
Transcription Factor Binding and Induced Transcription Alter Chromosomal c-myc Replicator Activity.
M. Ghosh, G. Liu, G. Randall, J. Bevington, and M. Leffak (2004)
Mol. Cell. Biol. 24, 10193-10207
   Abstract »    Full Text »    PDF »
Nuclear Factor 1 and Octamer Transcription Factor 1 Binding Preset the Chromatin Structure of the Mouse Mammary Tumor Virus Promoter for Hormone Induction.
S. Belikov, P.-H. Holmqvist, C. Astrand, and O. Wrange (2004)
J. Biol. Chem. 279, 49857-49867
   Abstract »    Full Text »    PDF »
{alpha}-Amanitin Blocks Translocation by Human RNA Polymerase II.
X. Q. Gong, Y. A. Nedialkov, and Z. F. Burton (2004)
J. Biol. Chem. 279, 27422-27427
   Abstract »    Full Text »    PDF »
Sequence and Spacing of TATA Box Elements Are Critical for Accurate Initiation from the {beta}-Phaseolin Promoter.
M. L. Grace, M. B. Chandrasekharan, T. C. Hall, and A. J. Crowe (2004)
J. Biol. Chem. 279, 8102-8110
   Abstract »    Full Text »    PDF »
Signal transduction pathways that inhibit hepatitis B virus replication.
M. D. Robek, B. S. Boyd, S. F. Wieland, and F. V. Chisari (2004)
PNAS 101, 1743-1747
   Abstract »    Full Text »    PDF »
Down-regulation of nuclear protein ICBP90 by p53/p21Cip1/WAF1-dependent DNA-damage checkpoint signals contributes to cell cycle arrest at G1/S transition.
Y. Arima, T. Hirota, C. Bronner, M. Mousli, T. Fujiwara, S.-i. Niwa, H. Ishikawa, and H. Saya (2004)
Genes Cells 9, 131-142
   Abstract »    Full Text »    PDF »
Differentiation-induced Colocalization of the KH-type Splicing Regulatory Protein with Polypyrimidine Tract Binding Protein and the c-src Pre-mRNA.
M. P. Hall, S. Huang, and D. L. Black (2004)
Mol. Biol. Cell 15, 774-786
   Abstract »    Full Text »    PDF »
RNA-Directed Actions of 8-Chloro-Adenosine in Multiple Myeloma Cells.
C. M. Stellrecht, C. O. Rodriguez Jr., M. Ayres, and V. Gandhi (2003)
Cancer Res. 63, 7968-7974
   Abstract »    Full Text »    PDF »
Lipopolysaccharide Induces Anandamide Synthesis in Macrophages via CD14/MAPK/Phosphoinositide 3-Kinase/NF-{kappa}B Independently of Platelet-activating Factor.
J. Liu, S. Batkai, P. Pacher, J. Harvey-White, J. A. Wagner, B. F. Cravatt, B. Gao, and G. Kunos (2003)
J. Biol. Chem. 278, 45034-45039
   Abstract »    Full Text »    PDF »
RNA Polymerase III Transcripts and the PTB Protein Are Essential for the Integrity of the Perinucleolar Compartment.
C. Wang, J. C. Politz, T. Pederson, and S. Huang (2003)
Mol. Biol. Cell 14, 2425-2435
   Abstract »    Full Text »    PDF »
Two Time Periods of Hippocampal mRNA Synthesis Are Required for Memory Consolidation of Fear-Motivated Learning.
L. M. Igaz, M. R. M. Vianna, J. H. Medina, and I. Izquierdo (2002)
J. Neurosci. 22, 6781-6789
   Abstract »    Full Text »    PDF »
Transcription-coupled and DNA damage-dependent ubiquitination of RNA polymerase II in vitro.
K.-B. Lee, D. Wang, S. J. Lippard, and P. A. Sharp (2002)
PNAS 99, 4239-4244
   Abstract »    Full Text »    PDF »
Tumor Genotype-specific Growth Inhibition in Vivo by Antisense Oligonucleotides against a Polymorphic Site of the Large Subunit of Human RNA Polymerase II.
K. Fluiter, A. L. M. A. ten Asbroek, M. van Groenigen, M. Nooij, M. C. G. Aalders, and F. Baas (2002)
Cancer Res. 62, 2024-2028
   Abstract »    Full Text »    PDF »
Inhibition of RNA transcription modulates magnesium-supplemented potassium cardioplegia protection.
H. Matsuda, S. Levitsky, and J. D. McCully (2000)
Ann. Thorac. Surg. 70, 2107-2112
   Abstract »    Full Text »    PDF »
Creb-Binding Protein (Cbp/P300) and RNA Polymerase II Colocalize in Transcriptionally Active Domains in the Nucleus.
A. von Mikecz, S. Zhang, M. Montminy, E. M. Tan, and P. Hemmerich (2000)
J. Cell Biol. 150, 265-274
   Abstract »    Full Text »    PDF »
Functional association of nuclear protein 4.1 with pre-mRNA splicing factors.
M. Lallena, C Martinez, J Valcarcel, and I Correas (2000)
J. Cell Sci. 111, 1963-1971
   Abstract »    PDF »
Nucleolar Localization of Human Methionyl-Trna Synthetase and Its Role in Ribosomal RNA Synthesis.
Y.-G. Ko, Y.-S. Kang, E.-K. Kim, S. G. Park, and S. Kim (2000)
J. Cell Biol. 149, 567-574
   Abstract »    Full Text »    PDF »
Human Transaldolase-associated Repetitive Elements Are Transcribed by RNA Polymerase III.
A. Perl, E. Colombo, E. Samoilova, M. C. Butler, and K. Banki (2000)
J. Biol. Chem. 275, 7261-7272
   Abstract »    Full Text »    PDF »
Polymorphisms in the large subunit of human RNA polymerase II as target for allele-specific inhibition.
A. L. M. A. ten Asbroek, K. Fluiter, M. v. Groenigen, M. Nooij, and F. Baas (2000)
Nucleic Acids Res. 28, 1133-1138
   Abstract »    Full Text »    PDF »
Control of Gene Expression at the Onset of Bovine Embryonic Development.
E. Memili and N. L. First (1999)
Biol Reprod 61, 1198-1207
   Abstract »    Full Text »
Detection and Identification of Amanitins in the Wood-Rotting Fungi Galerina fasciculata and Galerina helvoliceps.
S. Muraoka, N. Fukamachi, K. Mizumoto, and T. Shinozawa (1999)
Appl. Envir. Microbiol. 65, 4207-4210
   Abstract »    Full Text »
Effect of Prolactin on the Expression of Luteinizing Hormone Receptors during Cell Differentiation in Cultured Rat Granulosa Cells.
T. Hirakawa, T. Minegishi, M. Tano, T. Kameda, H. Kishi, Y. Ibuki, T. Mizutani, and K. Miyamoto (1999)
Endocrinology 140, 3444-3451
   Abstract »    Full Text »
The Transcriptional Inhibitors, Actinomycin D and alpha -Amanitin, Activate the HIV-1 Promoter and Favor Phosphorylation of the RNA Polymerase II C-terminal Domain.
C. Casse, F. Giannoni, V. T. Nguyen, M.-F. Dubois, and O. Bensaude (1999)
J. Biol. Chem. 274, 16097-16106
   Abstract »    Full Text »    PDF »
The Perinucleolar Compartment and Transcription.
S. Huang, T. J. Deerinck, M. H. Ellisman, and D. L. Spector (1998)
J. Cell Biol. 143, 35-47
   Abstract »    Full Text »    PDF »
Estrogen Regulation of Peptidylglycine {alpha}-Amidating Monooxygenase Messenger Ribonucleic Acid Levels by a Nuclear Posttranscriptional Event.
R. El Meskini, F. Boudouresque, and L'H. Ouafik (1997)
Endocrinology 138, 5256-5265
   Abstract »    Full Text »    PDF »
The Tax Protein of Human T-cell Leukemia Virus Type 1 Mediates the Transactivation of the c-sis/Platelet-derived Growth Factor-B Promoter through Interactions with the Zinc Finger Transcription Factors Sp1 and NGFI-A/Egr-1.
S. R. Trejo, W. E. Fahl, and L. Ratner (1997)
J. Biol. Chem. 272, 27411-27421
   Abstract »    Full Text »    PDF »
A Transcription-Dependent Switch Controls Competence of Adult Neurons for Distinct Modes of Axon Growth.
D. S. Smith and J. H. Pate Skene (1997)
J. Neurosci. 17, 646-658
   Abstract »    Full Text »    PDF »
Transcription-dependent redistribution of nuclear protein 4.1 to SC35-enriched nuclear domains.
M. Lallena and I Correas (1997)
J. Cell Sci. 110, 239-247
   Abstract »    PDF »
Ret 4, a Positive Acting Rhodopsin Regulatory Element Identified Using a Bovine Retina in Vitro Transcription System.
S. Chen and D. J. Zack (1996)
J. Biol. Chem. 271, 28549-28557
   Abstract »    Full Text »    PDF »
Amanitin Greatly Reduces the Rate of Transcription by RNA Polymerase II Ternary Complexes but Fails to Inhibit Some Transcript Cleavage Modes.
M. D. Rudd and D. S. Luse (1996)
J. Biol. Chem. 271, 21549-21558
   Abstract »    Full Text »    PDF »
Roles of Vaccinia Virus Ribonucleotide Reductase and Glutaredoxin in DNA Precursor Biosynthesis.
I. Rajagopal, B.-Y. Ahn, B. Moss, and C. K. Mathews (1995)
J. Biol. Chem. 270, 27415-27418
   Abstract »    Full Text »    PDF »
Action of alpha-Amanitin during Pyrophosphorolysis and Elongation by RNA Polymerase II.
D. R. Chafin, H. Guo, and D. H. Price (1995)
J. Biol. Chem. 270, 19114-19119
   Abstract »    Full Text »    PDF »
Dynamics of Transcription and Pre-mRNA Splicing within the Mammalian Cell Nucleus.
D.L. Spector, R.T. O'Keefe, and L.F. Jimenez-Garcia (1993)
Cold Spring Harb Symp Quant Biol 58, 799-805
   Abstract »    PDF »
Equipotent mouse ribosomal protein promoters have a similar architecture that includes internal sequence elements..
N Hariharan, D E Kelley, and R P Perry (1989)
Genes & Dev. 3, 1789-1800
   Abstract »    PDF »
A critical period for macromolecular synthesis in long-term heterosynaptic facilitation in Aplysia.
P. Montarolo, P Goelet, V. Castellucci, J Morgan, E. Kandel, and S Schacher (1986)
Science 234, 1249-1254
   Abstract »    PDF »
Participation of c-myc protein in DNA synthesis of human cells.
G. Studzinski, Z. Brelvi, S. Feldman, and R. Watt (1986)
Science 234, 467-470
   Abstract »    PDF »
Mushroom Poisoning: Case Reports and a Review of Therapy.
J. P. Hanrahan and M. A. Gordon (1984)
JAMA 251, 1057-1061
   Abstract »    PDF »
Mammary Tumor Virus DNA: A Glucocorticoid-responsive Transposable Element.
K. R. Yamamoto, M. R. Stallcup, J. Ring, and G. M. Ringold (1978)
Cold Spring Harb Symp Quant Biol 42, 625-638
   Abstract »    PDF »
Ornithine decarboxylase may function as an initiation factor for RNA polymerase I.
C Manen and D. Russel (1977)
Science 195, 505-506
   Abstract »    PDF »
The Transcriptional Role of Host DNA-dependent RNA Polymerases in Adenovirus-infected KB Cells.
R. Weinmann, H. J. Raskas, and R. G. Roeder (1974)
Cold Spring Harb Symp Quant Biol 39, 495-499
   Abstract »    PDF »
Studies on the Structure and Synthesis of Rous Sarcoma Virus RNA.
C. Weissmann, J. T. Parsons, J. W. Coffin, L. Rymo, M. A. Billeter, and H. Hofstetter (1974)
Cold Spring Harb Symp Quant Biol 39, 1043-1056
   Abstract »    PDF »
The Non symbiotic Origin of Mitochondria.
R. A. Raff and H. R. Mahler (1972)
Science 177, 575-582
   PDF »
Identification and Characterization of Human DNA Polymerase beta 2, a DNA Polymerase beta -Related Enzyme.
K.-i. Nagasawa, K. Kitamura, A. Yasui, Y. Nimura, K. Ikeda, M. Hirai, A. Matsukage, and M. Nakanishi (2000)
J. Biol. Chem. 275, 31233-31238
   Abstract »    Full Text »    PDF »
Cloning and Characterization of MDDX28, a Putative DEAD-box Helicase with Mitochondrial and Nuclear Localization.
R. Valgardsdottir, G. Brede, L. G. Eide, E. Frengen, and H. Prydz (2001)
J. Biol. Chem. 276, 32056-32063
   Abstract »    Full Text »    PDF »
Transcription-coupled and DNA damage-dependent ubiquitination of RNA polymerase II in vitro.
K.-B. Lee, D. Wang, S. J. Lippard, and P. A. Sharp (2002)
PNAS 99, 4239-4244
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)