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Science 16 October 1970:
Vol. 170. no. 3955, pp. 332 - 333
DOI: 10.1126/science.170.3955.332
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Articles

Hemagglutination Assay for Antigen and Antibody Associated with Viral Hepatitis

G. N. Vyas 1 and N. R. Shulman 2

1 Department of Clinical Pathology and Laboratory Medicine, University of California School of Medicine, San Francisco 94122
2 Clinical Hematology Branch, National Institute of Arthritis and Metabolic Diseases, Bethesda, Maryland 20014

Hemagglutination assays are described for measuring hepatitis-associated Australia antigen and antibody. Red cells coated with isolated antigen, with chromic chloride as a coupling agent, are used for detection of antibodies. Detection of the antigen in serums depends on inhibition of hemagglutination. The test has the sensitivity and rapidity of the best tests available, is simpler to perform, and lends itself to large-scale screening of blood donors.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Post-transfusion Hepatitis: Current Perspectives.
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Passive-Active Immunity from Hepatitis B Immune Globulin: Reanalysis of a Veterans Administration Cooperative Study of Needle-Stick Hepatitis.
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e Antigen in Hepatitis B Virus Infected Dialysis Patients: Assessment of Its Prognostic Value.
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Ann Intern Med 88, 285-293
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Transmission of Non-A, Non-B Hepatitis.
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Ann Intern Med 86, 272-277
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Hepatitis B Surface Antigen Carrier State in Neonates: Prophylaxis With Large Doses of Conventional Immune Human Serum Globulin.
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The Liver and the Antigens of Hepatitis B.
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Hepatitis B Surface Antigen and Antibody in Asymptomatic Blood Donors.
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JAMA 234, 1135-1138
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Ann Intern Med 83, 489-495
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Hepatitis Types B and Non-B: Epidemiologic Background.
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JAMA 233, 967-969
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Chronic Membranous Glomerulonephritis Caused by Hepatitis B Antigen-Antibody Immune Complexes.
P. F. KOHLER, R. E. CRONIN, W. S. HAMMOND, D. OLIN, and R. I. CARR (1974)
Ann Intern Med 81, 448-451
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Hepatitis B Antigen and Antibody: Occurrence in Families of Asymptomatic HB Ag Carriers.
G. R. Irwin, A. M. Allen, W. H. Bancroft, J. J. Karwacki, R. H. Pinkerton, and P. K. Russell (1974)
JAMA 227, 1042-1043
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Hepatitis B Transmission in a Family.
W. E. Mitch, J. R. Wands, and W. C. Maddrey (1974)
JAMA 227, 1043-1044
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Hepatitis B Infection: A Point-Prevalence Study in 15 US Hemodialysis Centers.
W. Szmuness, A. M. Prince, G. F. Grady, M. K. Mann, R. W. Levine, E. A. Friedman, M. J. Jacobs, A. Josephson, S. Ribot, F. L. Shapiro, et al. (1974)
JAMA 227, 901-906
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Antibody Responses in Viral Hepatitis, Type B.
L. F. Barker, M. R. Peterson, N. R. Shulman, and R. Murray (1973)
JAMA 223, 1005-1008
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Australia Antigen (Hepatitis B Antigen): A Conformational Antigen Dependent on Disulfide Bonds.
G. N. Vyas, K. R. Rao, and A. B. Ibrahim (1972)
Science 178, 1300-1301
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Posttransfusion Hepatitis After Exclusion of Commercial and Hepatitis-B Antigen-Positive Donors.
H. J. ALTER, P. V. HOLLAND, R. H. PURCELL, J. J. LANDER, S. M. FEINSTONE, A. G. MORROW, and P. J. SCHMIDT (1972)
Ann Intern Med 77, 691-699
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Collagen Diseases, Hepatitis-Associated Antigen, and The Epstein-Barr Virus.
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JAMA 221, 1517-1518
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Antibody to Hepatitis-Associated Antigen: Frequency and Pattern of Response as Detected by Radioimmunoprecipitation.
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JAMA 220, 1079-1082
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Coincidental Australia Antigenemia in Primary Biliary Cirrhosis.
W. C. MADDREY, S. SAITO, N. R. SHULMAN, and G. KLATSKIN (1972)
Ann Intern Med 76, 705-709
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Epidemiology of Infections With Type B Virus.
R. W. McCollum (1972)
Arch Pediatr Adolesc Med 123, 364-367
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Australia Antigen and Polyarteritis Nodosa.
C. G. Trepo, J. Thivolet, and A. M. Prince (1972)
Arch Pediatr Adolesc Med 123, 390-392
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Immune Response in Patients Who Received Blood Containing Serum Hepatitis Antigen.
A. M. Prince, B. Brotman, and C. E. Cherubin (1972)
Arch Pediatr Adolesc Med 123, 415-418
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A Prospective Study of Posttransfusion Hepatitis: The Role of Australia Antigen.
D. J. Gocke (1972)
JAMA 219, 1165-1170
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Hepatoma and HAA.
B. D. TEPFER (1972)
Ann Intern Med 76, 145-146
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Viral Hepatitis, Type B (MS-2 Strain) Prevention With Specific Hepatitis B Immune Serum Globulin.
S. Krugman, J. P. Giles, and J. Hammond (1971)
JAMA 218, 1665-1670
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Viral Hepatitis, Type B (MS-2 Strain) Studies On Active Immunization.
S. Krugman, J. P. Giles, and J. Hammond (1971)
JAMA 217, 41-45
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Australia Antigen Ecology.
L. F. BARKER and S. T. GIBSON (1971)
Ann Intern Med 75, 130-132
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