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Science 25 September 1970:
Vol. 169. no. 3952, pp. 1322 - 1324
DOI: 10.1126/science.169.3952.1322
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Articles

Changes in Extrinsic Fluorescence in Squid Axons during Voltage-Clamp

Franco Conti 1 and Ichiji Tasaki 1

1 Laboratorio di Cibernetica e Biofisica CNR, Corso Mazzini 72, Camogli 16032, Italy

Fluorescence changes in squid axons were examined after staining with rhodamine B, pyronin B, or 8-anilinonaphthalene-1-sulfonate by intracellular application. Gradual changes in fluorescence were detected during both hyperpolarizing and depolarizing voltage-clamp pulses. Abrupt changes were often observed at the onset and at the end of voltage-clamp. Possible sources of artifact in optical measurements of this type and some implications of the findings are discussed.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)