Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 28 August 1970:
Vol. 169. no. 3948, pp. 880 - 882
DOI: 10.1126/science.169.3948.880
Prev | Table of Contents | Next

Articles

Desmosterol as the Major Sterol in L-Cell Mouse Fibroblasts Grown in Sterol-Free Culture Medium

G. H. Rothblat 1, C. H. Burns 1, R. L. Conner 2, and J. R. Landrey 2

1 Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania
2 Department of Biology, Bryn Mawr College, Bryn Mawr, Pennsylvania

The principal sterol synthesized by L-cell mouse fibroblasts is desmosterol. Cholesterol was not detected in these cells when they were grown in a sterol-free culture medium. These findings indicate that, in cells, cholesterol can be replaced by desmosterol. Sterol analyses of six other tissue culture cell lines revealed cholesterol synthesis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Studies on the Cholesterol-Free Mouse: Strong Activation of LXR-Regulated Hepatic Genes When Replacing Cholesterol With Desmosterol.
M. Heverin, S. Meaney, A. Brafman, M. Shafir, M. Olin, M. Shafaati, S. von Bahr, L. Larsson, A. Lovgren-Sandblom, U. Diczfalusy, et al. (2007)
Arterioscler. Thromb. Vasc. Biol. 27, 2191-2197
   Abstract »    Full Text »    PDF »
Dual roles for cholesterol in mammalian cells.
F. Xu, S. D. Rychnovsky, J. D. Belani, H. H. Hobbs, J. C. Cohen, and R. B. Rawson (2005)
PNAS 102, 14551-14556
   Abstract »    Full Text »    PDF »
Generation of Viable Cholesterol-Free Mice.
A. Wechsler, A. Brafman, M. Shafir, M. Heverin, H. Gottlieb, G. Damari, S. Gozlan-Kelner, I. Spivak, O. Moshkin, E. Fridman, et al. (2003)
Science 302, 2087
   Full Text »    PDF »
Inhibition of sonic hedgehog autoprocessing in cultured mammalian cells by sterol deprivation.
R. K. Guy (2000)
PNAS 97, 7307-7312
   Abstract »    Full Text »    PDF »
Cellular Cholesterol Efflux Mediated by Cyclodextrins.
E. P. C. Kilsdonk, P. G. Yancey, G. W. Stoudt, F. W. Bangerter, W. J. Johnson, M. C. Phillips, and G. H. Rothblat (1995)
J. Biol. Chem. 270, 17250-17256
   Abstract »    Full Text »    PDF »
Desmosterol in Human and Experimental Brain Tumors in Tissue Culture.
J. F. Weiss, H. Cravioto, K. Bennett, E. deC. Weiss, and J. Ransohoff (1976)
Arch Neurol 33, 180-182
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)