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Science 24 July 1970:
Vol. 169. no. 3943, pp. 373 - 374
DOI: 10.1126/science.169.3943.373
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Articles

Puffing and Histone Acetylation in Polytene Chromosomes

Ulrich Clever 1 and Erik G. Ellgaard 1

1 Department of Biological Sciences, Purdue University, Lafayette, Indiana

Fixation with picric acid or formaldehyde retains incorporated [3H]acetate which is lost after fixation with ethanol and acetic acid. Unlike [3H]uridine, [3H]acetate is diffusely incorporated into polytene chromosomes, and not preferentially into existing or newly induced puffs. It is suggested that puff formation does not include an acetylation of histones.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)