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Science 26 June 1970:
Vol. 168. no. 3939, pp. 1592 - 1595
DOI: 10.1126/science.168.3939.1592
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Articles

Bulk Isolation in Nonaqueous Media of Nuclei from Lyophilized Cells

Wolff M. Kirsch 1, John W. Leitner 1, Michael Gainey 1, Demoy Schulz 1, Robert Lasher 1, and Paul Nakane 1

1 Departments of Surgery (Neurosurgery), Pathology, and Anatomy, University of Colorado Medical Center, Denver

Intact lyophilized nuclei are obtainable from a variety of tissues, either in situ or in culture, by freezing at -156°C, drying at -25°C, and mechanical disassociation in glycerol at 2°C. Centrifugal separation of nuclei is accomplished in an 85 : 15 by volume mixture of glycerol and 3-chloro-1,2 propanediol at 2°C. The method gives homogeneous nuclear preparations in high yield with preservation of labile and water-soluble constituents.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)