Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 5 June 1970:
Vol. 168. no. 3936, pp. 1220 - 1223
DOI: 10.1126/science.168.3936.1220
Prev | Table of Contents | Next

Articles

Experimental Allergic Encephalomyelitis: Synthesis of Disease-Inducing Site of the Basic Protein

E. H. Eylar 1, Juanita Caccam 1, J. J. Jackson 1, Fred C. Westall 2, and Arthur B. Robinson 3

1 Salk Institute, San Diego, California 92112
2 Department of Chemistry, University of California, San Diego
3 Department of Biology, University of California, San Diego

A highly encephalitogenic peptide whose structure resembles the sequence of amino acids surrounding the single tryptophan residue in the encephalitogenic A1 protein from bovine myelin was synthesized. This peptide is similar in the sequence to peptic peptide E and tryptic T27, derived directly from the A1 protein, and is as active on a molar basis as the A1 protein. The major disease-inducing site of the A1 protein resides in a linear sequence of nine amino acids: H-Phe-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH. This region of the A1 protein is apparently the major encephalitogenic determinant since specific modification of the tryptophan residue in the A1 protein with 2-hydroxy-5-nitrobenzyl bromide destroyed its encephalitogenic activity.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Molecular mimicry revisited: gut bacteria and multiple sclerosis..
F. C. Westall (2006)
J. Clin. Microbiol. 44, 2099-2104
   Abstract »    Full Text »    PDF »
Acinetobacter Immune Responses in Multiple Sclerosis: Etiopathogenetic Role and Its Possible Use as a Diagnostic Marker.
A. Ebringer, L. Hughes, T. Rashid, and C. Wilson (2005)
Arch Neurol 62, 33-36
   Abstract »    Full Text »    PDF »
Antibody Responses to Acinetobacter spp. and Pseudomonas aeruginosa in Multiple Sclerosis: Prospects for Diagnosis Using the Myelin-Acinetobacter-Neurofilament Antibody Index.
L. E. Hughes, S. Bonell, R. S. Natt, C. Wilson, H. Tiwana, A. Ebringer, P. Cunningham, V. Chamoun, E. J. Thompson, J. Croker, et al. (2001)
Clin. Vaccine Immunol. 8, 1181-1188
   Abstract »    Full Text »    PDF »
Autoantibodies to Brain Components and Antibodies to Acinetobacter calcoaceticus Are Present in Bovine Spongiform Encephalopathy.
H. Tiwana, C. Wilson, J. Pirt, W. Cartmell, and A. Ebringer (1999)
Infect. Immun. 67, 6591-6595
   Abstract »    Full Text »    PDF »
Experimental allergic encephalomyelitis in Lewis rats: chemical synthesis of disease-inducing determinant.
G. Hashim (1977)
Science 196, 1219-1221
   Abstract »    PDF »
Myelin Basic Protein: Location of Multiple Independent Antigenic Regions.
B. F. Driscoll, A. J. Kramer, and M. W. Kies (1974)
Science 184, 73-75
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)