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Science 17 April 1970:
Vol. 168. no. 3929, pp. 387 - 389
DOI: 10.1126/science.168.3929.387
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Articles

Murine Sarcoma Virus: The Question of Defectiveness

Robertson Parkman 1, Jay A. Levy 2, and Robert C. Ting 3

1 Laboratory of Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
2 Viral Carcinogenesis Branch, National Cancer Institute
3 Laboratory of Biology, National Cancer Institute

Infection of mouse and rat cells by the murine sarcoma virus (Moloney isolate) showed two-hit kinetics for focus production in mouse cells but one-hit kinetics in rat cells. Antiserum added to cultures after infection suppressed focus formation in mouse cells but not in rat cells. These studies suggest that, in rat cells infected with murine sarcoma virus, leukemina virus is not needed for focus formation and that these foci result from proliferation of the transformed rat cell; in mouse cells, on the other hand, leukemia virus is needed as "helper," and focus formation requires spread of virus. The term "defectiveness" then, if used, should not be applied to RNA tumor viruses without qualification for the viral function studied and the cell system employed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Xenotropic Viruses: Murine Leukemia Viruses Associated with NIH Swiss, NZB, and Other Mouse Strains.
J. A. Levy (1973)
Science 182, 1151-1153
   Abstract »    PDF »
Demonstration of Biological Activity of a Murine Leukemia Virus of New Zealand Black Mice.
J. A. Levy and T. Pincus (1970)
Science 170, 326-327
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)