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Science 10 April 1970:
Vol. 168. no. 3928, pp. 255 - 257
DOI: 10.1126/science.168.3928.255
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Articles

Inside-Out Red Cell Membrane Vesicles: Preparation and Puirification

Theodore L. Steck 1, Ronald S. Weinstein 2, John H. Straus 3, and Donald F. H. Wallach 3

1 Biochemical Research Laboratory, Massachusetts General Hospital, and Harvard Medical School, Boston 02114
2 Department of Pathology, Massachusetts General Hospital, and Harvard Medical School
3 Biochemical Research Laboratory, Massachusetts General Hospital, and and Harvard Medical School

Plasma membranes purified from human red cells were Converted into small vesicleS by disruption in alkaline buffer of low ionic strength. Most of these vesicles were inside-out. The presence of divalent cations prevented this inversion. The inside-out vesicles were separatcd from right-side-out vesicles by centrifugration to equilibrium in dextran density gradients.


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