Growth in vitro of Cells from Hyperplastic Nodules of Liver Induced by 2-Fluorenylacetamide or Aflatoxin B1

doi:10.1126/science.167.3916.285

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Science 16 January 1970:
Vol. 167. no. 3916, pp. 285 - 287
DOI: 10.1126/science.167.3916.285

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Growth in vitro of Cells from Hyperplastic Nodules of Liver Induced by 2-Fluorenylacetamide or Aflatoxin B₁

Malcolm Slifkin ¹, Leonard P. Merkow ¹, Matias Pardo ¹, Sheldon M. Epstein ², Joseph Leighton ², and Emmanuel Farber ²

¹ William H. Singer Menmorial Research Iitstitiute Of the Allegheny General Hospital, Pittsburgh, Pennsylania
² Department of Pathology, University, of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213

Cell suspensions obtained from hyperplastic nodules inducedin rat liver by either of the two hepatic carcinogens, 2-fluorenylacetamideor aflatoxin B₁, show growth when cultured in vitro. No growthof cells from liver adjacent to the hyperplastic nodules orfrom liver of control rats has been obtained so far under comparableconditions. Hepatocarcinoma cells induced by 2-fluorenylacetamidegrow readily in vitro but behave differently. These findingssuggest that some nonmalignant cells capable of growth in vitroarise during liver carcinogenesis prior to the appearance ofunequivocal cancer. Cultures of such cells may offer new avenuesfor the study of liver carcinogenesis.

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