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Science 16 January 1970:
Vol. 167. no. 3916, pp. 281 - 283
DOI: 10.1126/science.167.3916.281
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Articles

Countercurrent Chromatography: Liquid-Liquid Partition Chromatography without Solid Support

Yoichiro Ito 1 and Robert L. Bowman 1

1 Laboratory of Technical Development, National Heart Institute, Bethesda, Maryland 20014

The liquid-liquid partition chromatographic system reported here involves a long helix of narrow-bore tubing. When the coiled tube is filled with one phase of a two-phase system and fed with the other phase, phase-interchange takes place in each turn of the coil, leaving a segment of the former phase as the stationary phase. Consequently, solutes present in either phase are subjected to a multistep partition process. The column efficiency, estimated on a separation of dinitrophenyl amino acids, is comparable to that of gas chromatography.


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