Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 5 December 1969:
Vol. 166. no. 3910, pp. 1280 - 1282
DOI: 10.1126/science.166.3910.1280
Prev | Table of Contents | Next

Articles

Plus and Minus Single-Stranded DNA Separately Encapsidated in Adeno-Associated Satellite Virions

Heather D. Mayor 1, Katsutaka Torikai 1, Joseph L. Melnick 1, and Manley Mandel 2

1 Baylor College of Medicine, Houston, Texas 77025
2 University of Texas M. D. Anderson Hospital atnd Tumor Institute at Houston. Houston 77025

Based on physical and chemical determinations, the mnolecular weight of the type 4 adeno-satellite virus is 5.4 X 106 daltons, and the virion contains 1.4 X 106 daltons of DNA. Denaturation and renaturation studies indicate that the viral genome is a single-stranded DNA molecule and that each virion contains either a minus or a plus strand. Upon extraction, the minus and plus strands unite to form double-stranded DNA molecules with no obvious excess of unpaired strands.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cloning of an Avian Adeno-Associated Virus (AAAV) and Generation of Recombinant AAAV Particles.
I. Bossis and J. A. Chiorini (2003)
J. Virol. 77, 6799-6810
   Abstract »    Full Text »    PDF »
Biochemical Characterization of Junonia coenia Densovirus Nonstructural Protein NS-1.
C. Ding, M. Urabe, M. Bergoin, and R. M. Kotin (2002)
J. Virol. 76, 338-345
   Abstract »    Full Text »    PDF »
Adeno-associated Virus DNA Replication.
K. I. Berns, W. W. Hauswirth, K. H. Fife, and E. Lusby (1979)
Cold Spring Harb Symp Quant Biol 43, 781-787
   Abstract »    PDF »
Nonencapsidated Infectious DNA of Adeno-Satellite Virus in Cells Coinfected with Herpesvirus.
D. W. Boucher, J. L. Melnick, and H. D. Mayor (1971)
Science 173, 1243-1245
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)