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Science 24 October 1969:
Vol. 166. no. 3904, pp. 516 - 517
DOI: 10.1126/science.166.3904.516
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Articles

Nosema cuniculi: In vitro Isolation

John A. Shadduck 1

1 Department of Veterinary Pathology Ohlio State University, Columbus 43210

A mammalian microsporidian was propagated for the first time in vitro with rabbit choroid plexus monolayer cultures. Tentative classification as Nosema cuniculi (Levaditi, 1923) was established by morphologic and tinctorial characteristics and by extrusion of sintgle polar filaments with attached sporoplasin from living spores. The method permits propagation of large numbers of organisms in putre cultutre.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
In Vitro Cultivation of Microsporidia of Clinical Importance.
G. S. Visvesvara (2002)
Clin. Microbiol. Rev. 15, 401-413
   Abstract »    Full Text »    PDF »
Mammalian Microsporidiosis.
K. Wasson and R. L. Peper (2000)
Vet. Pathol. 37, 113-128
   Abstract »    Full Text »    PDF »
CD8+ CTLs Are Essential for Protective Immunity Against Encephalitozoon cuniculi Infection.
I. A. Khan, J. D. Schwartzman, L. H. Kasper, and M. Moretto (1999)
J. Immunol. 162, 6086-6091
   Abstract »    Full Text »    PDF »
Molecular Techniques for Detection, Species Differentiation, and Phylogenetic Analysis of Microsporidia.
C. Franzen and A. Muller (1999)
Clin. Microbiol. Rev. 12, 243-285
   Abstract »    Full Text »    PDF »
Role of Gamma Interferon in Cellular Immune Response against Murine Encephalitozoon cuniculi Infection.
I. A. Khan and M. Moretto (1999)
Infect. Immun. 67, 1887-1893
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)