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Science 12 September 1969:
Vol. 165. no. 3898, pp. 1128 - 1131
DOI: 10.1126/science.165.3898.1128
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Articles

Uptake of Isolated Chloroplasts by Mammalian Cells

Margit M. k. Nass 1

1 Department of Therapeutic Research, University of Pennsylvania School of Medicine, Philadelphia, 19104

Mouse fibroblasts (L cells) in suspension culture incorporated isolated chloroplasts of spinach and African violets and isolated mitochondria of chicken liver. The organelles resided in the cell cytoplasm and were not contained in vacuoles or digestion vesicles. Green cells divided like normal cells. Green chloroplasts were followed for five cell generations or 5 days, at which time hybrid cells were greatly outnumbered by nongreen progeny cells. The ingested chloroplasts retained their structural integrity as determined by electron microscopy of organelles and hybrid cells and by analysis of photochemical activity and DNA in chloroplasts reisolated from cells after 1 or 2 days in culture.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mollusc-Algal Chloroplast Endosymbiosis. Photosynthesis, Thylakoid Protein Maintenance, and Chloroplast Gene Expression Continue for Many Months in the Absence of the Algal Nucleus.
B. J. Green, W.-Y. Li, J. R. Manhart, T. C. Fox, E. J. Summer, R. A. Kennedy, S. K. Pierce, and M. E. Rumpho (2000)
Plant Physiology 124, 331-342
   Abstract »    Full Text »
Penetration of Somatic Mammalian Cells by Sperm.
A. Bendich, E. Borenfreund, and S. S. Sternberg (1974)
Science 183, 857-859
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)