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Science 11 April 1969:
Vol. 164. no. 3876, pp. 188 - 189
DOI: 10.1126/science.164.3876.188
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Articles

Immunological Detection of Single Amino Acid Substitutions in Alkaline Phosphatase

G. T. Cocks 1 and A. C. Wilson 1

1 Department of Biochemistry, University of California, Berkeley

Antiserums to wild-type alkaline phosphatase from Escherichia coli were prepared and tested for reactivity with phosphatases altered by point mutations. Eight out of the nine mutant enzymes were distinguished from the wild type with quantitative microcomplement fixation. The structural changes are among the smallest yet observed immunologically.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Metachromatic Leukodystrophy (MLD) X. Immunological Studies of the Abnormal Sulfatase A.
D. Stumpf, E. Neuwelt, J. Austin, and P. Kohler (1971)
Arch Neurol 25, 427-431
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Science. ISSN 0036-8075 (print), 1095-9203 (online)