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Science 14 March 1969:
Vol. 163. no. 3872, pp. 1213 - 1214
DOI: 10.1126/science.163.3872.1213
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Articles

Cell Microfluorometry: A Method for Rapid Fluorescence Measurement

M. A. Van Dilla 1, T. T. Truiullo 1, P. F. Mullaney 1, and J. R. Coultex 1

1 Biomedical Research Group, Los Alamos Scientific Laboratory, University of California, Los Alamos, New Mexico 87544

A high-speed flow system for quantitative determination of fluoresence of cells containing fluorochrome has been developed. Feulgen-DNA distributions in populations of tissue culture cells and human leukocytes havebeen measured at a rate of 104 to 105 cells per minute and compare well with results of other independent methods.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)