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Science 20 December 1968:
Vol. 162. no. 3860, pp. 1398 - 1400
DOI: 10.1126/science.162.3860.1398
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Articles

Affinity Site Labeling of a Mouse Myeloma Protein Which Binds Dinitrophenyl Ligands

Henry Metzger 1 and Michael Potter 2

1 National Institute of Arthritis and Metabolic Diseases, Bethesda, Maryland
2 National Cancer Institute, Bethesda, Maryland

A mouse myeloma protein of the immunoglobulin A class, which specifically binds dinitrophenyl ligands, has been successfully affinity labeled (site-directed labeling) with several diazonium reagents, leading to inactivation of the combining sites. The labeling reagents reacted only with tyrosine residues of light chain origin. The 7S subunit of the myeloma protein appears to contain only one reactive site.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Model-building Studies of Antigen-binding Sites: The Hapten-binding Site of MOPC-315.
E. A. Padlan, D. R. Davies, I. Pecht, D. Givol, and C. Wright (1977)
Cold Spring Harb Symp Quant Biol 41, 627-637
   Abstract »    PDF »
Antigenicity: Some Molecular Aspects.
M. Sela (1969)
Science 166, 1365-1374
   PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)