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Science 20 December 1968:
Vol. 162. no. 3860, pp. 1384 - 1387
DOI: 10.1126/science.162.3860.1384
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Articles

Single Crystals of Transfer RNA from Formylmethionine and Phenylalanine Transfer RNA's

Arnold Hampel 1, Mindaugas Labanauskas 2, Peter G. Connors 2, Leslie Kirkegard 3, Uttam L. Rajbhandary 4, Paul B. Sigler 5, and Robert M. Bock 6

1 Department of Biochemistry, University of Wisconsin, Madison
2 Laboratory of Biophysics, University of Wisconsin
3 Department of Biochemistry, University of Wisconsin
4 Institute for Enzyme Research, University of Wisconsin
5 Department of Biophysics, University of Chicago, Chicago, Illinois
6 Department of Biochemistry and Laboratory of Molecular Biology, University of Wisconsin

Reproducible conditions have been developed for crystallization of transfer RNA. The conditions may be applicable to many pure transfer RNA species since identical procedures (except for initial transfer-RNA concentration) yielded good crystals from both yeast and Escherichia coli transfer RNA. These crystals, which must be kept at temperatures below about 10°C and handled in vapor of controlled alcohol concentration, have been studied by x-ray crystallography. The availability of crystals of a nucleic acid opens a route for extending knowledge of the tertiary structure of transfer RNA and its relation to important biological functions.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
x Ray crystallography.
M S Smyth and J H J Martin (2000)
Mol. Pathol. 53, 8-14
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Structure of Ustilago maydis Killer Toxin KP6 alpha -Subunit. A MULTIMERIC ASSEMBLY WITH A CENTRAL PORE.
N. Li, M. Erman, W. Pangborn, W. L. Duax, C.-M. Park, J. Bruenn, and D. Ghosh (1999)
J. Biol. Chem. 274, 20425-20431
   Abstract »    Full Text »    PDF »
Crystalline Transfer RNA: The Three-Dimensional Patterson Function at 12-Angstrom Resolution.
S.-H. Kim and A. Rich (1969)
Science 166, 1621-1624
   Abstract »    PDF »
Structural studies on transfer RNA: crystallization of formylmethionine and leucine transfer RNA's..
J. D. Young, R. M. Bock, S. Nishimura, H. Ishikura, Y. Yamada, U. L. RajBhandary, M. Labanauskas, and P. G. Connors (1969)
Science 166, 1527-1528
   Abstract »    PDF »
X-ray Diffraction Studies of Crystalline Transfer RNA.
R. M. Bock, J. D. Young, M. Labanauskas, and P. G. Connors (1969)
Cold Spring Harb Symp Quant Biol 34, 149-152
   Abstract »    PDF »
Transfer RNA Crystals Studied by X-Ray Diffraction.
S.-H. Kim, P. Schofield, and A. Rich (1969)
Cold Spring Harb Symp Quant Biol 34, 153-159
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)