Conversion of Mesophilic to Psychrophilic Bacteria
Ronald H. Olsen 1 and
Eleanor S. Metcalf 1
1 Department of Microbiology, University of Michigan, Ann Arbor
The minimum temperature of growth of the mesophilic bacterium Pseudomonas aeruginosa has been significantly lowered from approximately 11° to 0°C. This shift in the minimum temperature of growth was accompanied by a corresponding decrease in the maximum temperature of growth. Transfer of this genetic characteristic by a transducing phage grown on a psychrophile or by ultraviolet mutagenesis was used to accomplish these shifts in range of growth temperature.