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Science 29 November 1968:
Vol. 162. no. 3857, pp. 1012 - 1014
DOI: 10.1126/science.162.3857.1012
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Articles

Nonspecific Staining: Its Control in Immunofluorescence Examination of Soil

B. B. Bohlool 1 and E. L. Schmidt 2

1 Department of Soil Science, University of Minnesota, St. Paul
2 Departments of Microbiology and Soil Science, University of Minnesota, Minneapolis

Gelatin preparations were used to treat soil slides prior to addition of fluorescent antibody. Nonspecific staining was avoided, with no detectable interference to specific staining. Gelatin-rhodamine conjugates served to counterstain as well as to prevent nonspecific staining.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)