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Science 25 October 1968:
Vol. 162. no. 3852, pp. 464 - 465
DOI: 10.1126/science.162.3852.464
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Articles

Prophage S2 Mutants in Haemophilus influenzae: A Technique for Their Production and Isolation

John W. Bendler 1 and Sol H. Goodgal 1

1 Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104

A procedure utilizing nitrosoguanidine has been developed to produce defective and temperature-sensitive mutants of prophage (S2) in lysogenic Haemophilus influenzae. The system should be generally applicable to all temperate phage systems. At saturating concentrations of phage DNA, more than 25 percent of recipient mutant lysogenic bacteria can be transformed to the wild type.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cloning and Sequencing of a Genomic Island Found in the Brazilian Purpuric Fever Clone of Haemophilus influenzae Biogroup Aegyptius.
G. McGillivary, A. P. Tomaras, E. R. Rhodes, and L. A. Actis (2005)
Infect. Immun. 73, 1927-1938
   Abstract »    Full Text »    PDF »
Bacteriophage HP2 of Haemophilus influenzae.
B. J. Williams, M. Golomb, T. Phillips, J. Brownlee, M. V. Olson, and A. L. Smith (2002)
J. Bacteriol. 184, 6893-6905
   Abstract »    Full Text »    PDF »


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