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Science 6 September 1968:
Vol. 161. no. 3845, pp. 1025 - 1027
DOI: 10.1126/science.161.3845.1025
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Articles

Neuronal Perikarya of Rat Brain Isolated by Zonal Centrifugation

Arthur L. Flangas 1 and Robert E. Bowman 1

1 Psychochemistry Unit, Regional Primate Research Center, University of Wisconsin, Madison 53706

Cerebral cortex and hippocampus of rat were disrupted in a specially designed tissue-press and prepared as a 2 percent suspension in 10 percent buffered Ficoll medium and fractionated by density-gradient centrifugation in the B XIV zonal rotor. The suspension was injected into a buffered 30 percent Ficoll, 58 percent sucrose discontinuous gradient previously loaded in the B XlV zonal rotor spinning at 3500 revolutions per minute. Intact neuronal perikarya were recovered as a discrete band in the dense sucrose zone of the gradient after centrifugation at 35,000 revolutions per minute for 45 minutes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Neuronal Soma and Whole Neuroglia of Rat Brain: A New Isolation Technique.
W. T. Norton and S. E. Poduslo (1970)
Science 167, 1144-1146
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Science. ISSN 0036-8075 (print), 1095-9203 (online)