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Science 23 August 1968:
Vol. 161. no. 3843, pp. 806 - 807
DOI: 10.1126/science.161.3843.806
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Articles

Deoxyribonucleic Acid Antibody: A Method to Detect Its Primary Interaction with Deoxyribonucleic Acid

Richard T. Wold 1, Frank E. Young 1, Eng M. Tan 1, and Richard S. Farr 1

1 Departments of Clinical Biology, Microbiology, and Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Antibody to DNA in human serums can be detected by the ammonium sulfate method. This sensitive and specific technique, which measures the primary interaction between DNA and antibody to DNA, is based on the observation that free DNA is soluble in 50-percent saturated ammonium sulfate whereas antibody-bound DNA is insoluble.


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