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Science 9 August 1968:
Vol. 161. no. 3841, pp. 586 - 589
DOI: 10.1126/science.161.3841.586
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Articles

Bioluminescence: pH Activity Profies of Related Luciferase Fractions

Neil Krieger 1 and J. W. Hastings 1

1 Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138

The 27, 000g supernatant from crude extracts of the dinoflagellate Gonyaulax can be fractionated into two components having luciferase activity that differ both in molecular weight (35, 000 and 150, 000) and in pH activity profile. The smaller component has activity over a broad range from pH 5 to 9, while the larger one is active only in the acid region. This clarifies the previous ambiguity in the literature regarding the optimum pH for the assay of luciferase.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Inaugural Article: Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species.
L. Liu and J. W. Hastings (2007)
PNAS 104, 696-701
   Abstract »    Full Text »    PDF »
Profile of J. Woodland Hastings.
T. H. Davis (2007)
PNAS 104, 693-695
   Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)