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Science 9 August 1968:
Vol. 161. no. 3841, p. 570
DOI: 10.1126/science.161.3841.570
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Articles

RNA-DNA Hybridization: Demonstration in a Mammalian System of Competition by Preincubation

W. Stuart Riggsby 1 and Virginia Merriam 1

1 Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830

Nonspecific intermolecular interactions obscure the interpretation of competition experiments involving hybridization of DNA and RNA in mammalian systems when the labeled RNA/DNA ratio is low. The effect of these interactions can be minimized by choosing a sufficiently high ratio of labeled RNA/DNA. No serious effect of nonspecific interactions is observed in bacteriophage systems, even at very low labeled RNA/DNA ratios.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
RNA Competition in RNA-DNA Hybridization Systems.
J. S. Roth (1968)
Science 162, 699-700
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Science. ISSN 0036-8075 (print), 1095-9203 (online)