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Science 19 July 1968:
Vol. 161. no. 3838, pp. 272 - 274
DOI: 10.1126/science.161.3838.272
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Articles

Histochemical Fluorescence after Application of Neurochemicals to Caudate Nucleus and Septal Area in vivo

Aryeh Routtenberg 1, John Sladek 1, and William Bondareff 1

1 Departments of Psychology and Anatomy, Northwestern University, Evanston, Illinois 60201

The movement of carbachol, norepinephrine, and dopamine from cannula sites in caudate nucleus and septal area of freely moving rats was traced by means of biogenic amine fluorescence. Fluorescent patterns seen after application of carbachol and norepinephrine to brain tissue did not appear to differ from controls. Three types of movement from the cannula site after administration of dopamine were observed. There was a spherical distribution approximately 2 millimeters in diameter. Fluorescence also followed axonal pathways in the orthodromic direction, suggesting that dopamine may have been transported by " axonal streaming " or by some other unknown mechanism in periaxonal spaces. Because fluorescence was present in both the ependymal lining and the choroid plexus, it was inferred that the cerebral ventricles were also involved in the movement of chemical. Any attempt to ascribe anatomical localization to behavioral effects resulting from chemical stimulation of the brain should take into account the widespread movement of chemicals after their local application to brain tissue.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Subfornical Organ: Site of Drinking Elicitation by Angiotensin II.
J. B. Simpson and A. Routtenberg (1973)
Science 181, 1172-1175
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Histochemical Fluorescence as an Index of Spread of Centrally Applied Neurochemicals.
R. B. Montgomery, G. Singer, A. Routtenberg, and W. Bondareff (1969)
Science 165, 1031-1032
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