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Science 12 April 1968:
Vol. 160. no. 3824, pp. 194 - 195
DOI: 10.1126/science.160.3824.194
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Articles

Antibody Formation: Initiation in "Nonresponder" Mice by Macrophage Synthetic Polypeptide RNA

Paul Pinchuck 1, Marvin Fishman 1, Frank L. Adler 1, and Paul H. Maurer 1

1 Department of Biochemistry, Jefferson Medical College, Philadelphia, Pennsylvania, and Public Health Research Institute, New York

The RNA extracted from normal peritoneal macrophages exposed to a linear, random synthetic polypeptide, Glu60Ala30Tyr10, initiated an immune response in C57B1/6J mice, although this strain responds very poorly to the antigen itself. From 10 to 150 micrograms of RNA obtained from mouse, rat, or rabbit macrophages was injected intraperitoneally into recipient mice, and specific antibody was detectable by passive hemagglutination 3 to 4 weeks later. Treatment of the RNA with ribonuclease destroyed its ability to initiate a specific immune response. The RNA contained by weight 0.02 percent of the (specific) antigen. The RNA obtained from cells incubated with a second polypeptide, Glu36Lys24Ala40, initiated a response specific for this polymer. This RNA even when incubated in vitro with Glu60Ala30Tyr10 failed to initiate antibody formation specific for Glu60Ala30Tyr10.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Antigenicity: Some Molecular Aspects.
M. Sela (1969)
Science 166, 1365-1374
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Science. ISSN 0036-8075 (print), 1095-9203 (online)