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Science 23 February 1968:
Vol. 159. no. 3817, pp. 884 - 885
DOI: 10.1126/science.159.3817.884
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Articles

Antigen Binding to Cells: Determination by Enzymic Fluorogenic Group Hydrolysis

Eli E. Sercarz 1 and Farrokh Modabber 1

1 Department of Bacteriology, University of California, Los Angeles 90024

A sensitive method for detecting cells containing antibody to beta-galactosidase has been devised. The enzyme attached to the cells containing antibody can hydrolyze a fluorogenic substrate and yield fluorescent products which are measured microphotofluorometrically. This method of detecting a few molecules of antibody is applicable to other enzyme antigen systems.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)