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Science 18 August 1967:
Vol. 157. no. 3790, pp. 817 - 819
DOI: 10.1126/science.157.3790.817
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Articles

Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA

Mary-Dell Chilton 1

1 Department of Genetics, University of Washington, Seattle

Under conditions favoring single-strand transformation, the two complementary strands of Bacillus subtilis DNA, separated by differential complexing with polyriboguanylic acid, have identical transforming activity. Moreover separated single strands, upon renaturation with unmarked (recipient) DNA, form heteroduplex molecules with similar double-strand transformin activity. These findings bear upon the mechanism of DNA integration.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)