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Science 11 August 1967:
Vol. 157. no. 3789, pp. 701 - 703
DOI: 10.1126/science.157.3789.701
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Articles

Replication of Viral RNA: RNA Synthetase from Escherichia coli Infected with Phage MS2 or Qbeta

Gunter Feix 1, Morton C. Schneider 1, Charles Weissmann 1, and Severo Ochoa 1

1 Department of Biochemistry, New York University School of Medicine, New York

An RNA synthetase is formed in Escherichia coli after infection with bacteriophages containing RNA. Specific annealing techniques revealed that, from the very outset of the reaction in vitro, the partially purified enzyme-template complex synthesizes parental-type plus strands, namely, MS2-RNA when isolated from cells infected with MS2 phage and Qbeta-RNA in the case of cells infected with Qbeta phage. This is in contrast to the situation found with Qbeta replicase primed with Qbeta-RNA, where the initial product is the complementary strand.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)