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Science 9 June 1967:
Vol. 156. no. 3780, pp. 1369 - 1371
DOI: 10.1126/science.156.3780.1369
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Articles

Substrate Binding Properties of Mutant and Wild-Type A Proteins of Escherichia coli Tryptophan Synthetase

John K. Hardman 1 and Charles Yanofsky 2

1 McCollum-Pratt Institute, Biology Department, Johns Hopkins Untiversity, Baltimore, Maryland
2 Department of Biological Sciences, Stanford University, Stanford, California

Most of the mutant A proteins studied appear to be similar to the normal enzyme both in their apparent conformation about the critical cysteine residues and their ability to bind substrate. Two mutant proteins, in which a glutamic acid or arginine residue is substituted for a glycine residue, do appear abnormal suggesting that these primary structural changes radically affect the conformation in regions at or near the site or sites of substrate binding.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)