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Science 19 May 1967:
Vol. 156. no. 3777, pp. 964 - 966
DOI: 10.1126/science.156.3777.964
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Articles

Autoradiographic Plaques for the Detection of Antibody Formation to Soluble Proteins by Single Cells

Edger Pick 1 and Joseph D. Feldman 1

1 Department of experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Single cells that synthesize antibody to soluble proteins can be detected and counted by an autoradiographic procedure. The method is based on the specific binding of I131-labeled antigen to the antidogy which has been produced by and has diffused around cells in an agarose medium and which has been precipitated by an antibody to gamma globulin. The antigen-binding zones appear as dark spots on high-speed x-ray film exposed to the dried agarose preparations.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)