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Science 30 December 1966: Vol. 154. no. 3757, pp. 1662 - 1665 DOI: 10.1126/science.154.3757.1662
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Articles
Nuclei from Rat Liver: Isolation Method That Combines Purity with High Yield
Gunter Blobel 1 and
Van R. Potter 1
1 McArdle Laboratory, University of Wisconsin,Madison University of Wisconsin, Madison
A procedure is described that gives almost quantitative separation of clean nuclei from a homogenate of rat liver. It is a modification of methods that use concentrated sucrose solutions rather than citric acid or detergents and, therefore, permits further quantitative fractionation of cytoplasmic components.
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