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Science 30 December 1966:
Vol. 154. no. 3757, pp. 1662 - 1665
DOI: 10.1126/science.154.3757.1662

Articles

Nuclei from Rat Liver: Isolation Method That Combines Purity with High Yield

Gunter Blobel 1 and Van R. Potter 1

1 McArdle Laboratory, University of Wisconsin,Madison University of Wisconsin, Madison

A procedure is described that gives almost quantitative separation of clean nuclei from a homogenate of rat liver. It is a modification of methods that use concentrated sucrose solutions rather than citric acid or detergents and, therefore, permits further quantitative fractionation of cytoplasmic components.


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