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Science 23 December 1966:
Vol. 154. no. 3756, pp. 1575 - 1577
DOI: 10.1126/science.154.3756.1575
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Articles

Cytochemistry of Synapses: Selective Staining for Electron Microscopy

Floyd E. Bloom 1 and George K. Aghajanian 1

1 Departments of Anatomy and Psychiatry, Yale University School of Medicine and Connecticut Mental Health Center, New Haven 06510

Material at synapses and in some synaptic vesicles becomes selectively stained when glutaraldehyde-fixed rat brain that has not been treated with osmium is stained with phosphotungstic acid. The material stained at synapses is distinct from the adjacent unstained synaptic membranes and has cytochemical properties of protein. The specialized spatial arrangement exhibited by this synaptic material suggests its close involvement in synaptic function.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Conical Electron Tomography of a Chemical Synapse: Polyhedral Cages Dock Vesicles to the Active Zone.
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Control of Synaptic Connection by Glutamate Receptor {delta}2 in the Adult Cerebellum.
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Protein Aggregation after Transient Cerebral Ischemia.
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Modification of Postsynaptic Densities after Transient Cerebral Ischemia: A Quantitative and Three-Dimensional Ultrastructural Study.
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J. Neurosci. 19, 1988-1997
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Assembly of Proteins to Postsynaptic Densities after Transient Cerebral Ischemia.
B.-R. Hu, M. Park, M. E. Martone, W. H. Fischer, M. H. Ellisman, and J. A. Zivin (1998)
J. Neurosci. 18, 625-633
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A Guide to the Synaptic Analysis of the Neuropil.
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Cold Spring Harb Symp Quant Biol 40, 1-16
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Osmiophilic Polymer Generation: Catalysis by Transition Metal Compounds in Ultrastructural Cytochemistry.
J. S. Hanker, W. A. Anderson, and F. E. Bloom (1972)
Science 175, 991-993
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Science. ISSN 0036-8075 (print), 1095-9203 (online)