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Science 2 December 1966:
Vol. 154. no. 3753, pp. 1186 - 1189
DOI: 10.1126/science.154.3753.1186
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Articles

Establishment of Four Functional, Clonal Strains of Animal Cells in Culture

Yosihiro Yasumura 1, Armen H. Tashjian Jr. 1, and Gordon H. Sato 1

1 Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts and Harvard School of Dental Medicine, Boston

The single-cell plating technique was used to develop four clonal cell lines that perform organ-specific functions after being serially cultured for prolonged periods. These strains include steroid-secreting Leydig cells, melanomacells that form pigment, and two strains from a hormone-secreting rat pituitary tumor. One of the cell lines from the pituitary tumor secretes growt hormone, while another line derived from the same tumor secretes a substance similar to adrenocorticotropic hormone.


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