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Science 7 October 1966:
Vol. 154. no. 3745, pp. 152 - 153
DOI: 10.1126/science.154.3745.152
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Articles

A Disulfide-Linked Collagenous Protein of Nematocyst Capsules

Richard Blanquet 1 and Howard M. Lenhoff 2

1 Laboratory for Quantitative Biology, University of Miami, Coral Gables, Florida, andDuke University Marine Laboratory, Beaufort,North Carolina; Laboratory for Quantitative Biology, University of Miami, Coral Gables, Florida 33124.
2 Laboratory for Quantitative Biology and Department of Biology, University of Miami, Coral Gables, Florida

The major protein component present in the capsule and thread of a sea anemone nematocyst consists of monomers of a collagen-like protein linked by disulfide bonds. Purified nematocyst capsules and threads are rich in hydroxyproline, and dissolved by disulfide reducing agents. Electrophoresis of the dissolved component on acrylamide gel results in a single detectable protein band.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Formation of the Apical Flaps in Nematocysts of Sea Anemones (Cnidaria: Actiniaria).
A. J. Reft, J. A. Westfall, and D. G. Fautin (2009)
Biol. Bull. 217, 25-34
   Abstract »    Full Text »    PDF »
Cnidarian internal stinging mechanism.
A. Schlesinger, E. Zlotkin, E. Kramarsky-Winter, and Y Loya (2009)
Proc R Soc B 276, 1063-1067
   Abstract »    Full Text »    PDF »
The Glycoprotein NOWA and Minicollagens Are Part of a Disulfidelinked Polymer That Forms the Cnidarian Nematocyst Wall.
S. Ozbek, E. Pokidysheva, M. Schwager, T. Schulthess, N. Tariq, D. Barth, A. G. Milbradt, L. Moroder, J. Engel, and T. W. Holstein (2004)
J. Biol. Chem. 279, 52016-52023
   Abstract »    Full Text »    PDF »
The Structure of the Cys-rich Terminal Domain of Hydra Minicollagen, Which Is Involved in Disulfide Networks of the Nematocyst Wall.
E. Pokidysheva, A. G. Milbradt, S. Meier, C. Renner, D. Haussinger, H. P. Bachinger, L. Moroder, S. Grzesiek, T. W. Holstein, S. Ozbek, et al. (2004)
J. Biol. Chem. 279, 30395-30401
   Abstract »    Full Text »    PDF »
Structure/Function Relationships in the Minicollagen of Hydra Nematocysts.
S. Ozbek, O. Pertz, M. Schwager, A. Lustig, T. Holstein, and J. Engel (2002)
J. Biol. Chem. 277, 49200-49204
   Abstract »    Full Text »    PDF »
Novel tools for the study of development, migration and turnover of nematocytes (cnidarian stinging cells).
J Weber (1995)
J. Cell Sci. 108, 403-412
   Abstract »    PDF »
Invertebrate collagens.
E Adams (1978)
Science 202, 591-598
   Abstract »    PDF »
Coelenterate Biology: Experimental Research.
H. M. Lenhoff, L. Muscatine, and L. V. Davis (1968)
Science 160, 1141-1146
   PDF »


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