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Science 16 September 1966:
Vol. 153. no. 3742, pp. 1390 - 1391
DOI: 10.1126/science.153.3742.1390
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Articles

Cilia Isolated from Tetrahymena after Membrane Stabilization by 1,5-Difluoro-2,4-Dinitrobenzene

Jon R. Culbertson 1

1 Department of Biological Science, Florida State University, Tallahassee 32306

The tendency for Tetrahymena pyriformis cells to lyse when they are centrifuged at speeds above 120g and then exposed to an ethanolic deciliating medium is inhibited by treatment with 1,5-difluoro-2,4-dinitrobenzene. This treatment facilitates greater yields and purer preparations of isolated cilia. Cilia from treated and untreated cells do not differ in some of their biochemical properties.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)