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Science 16 September 1966:
Vol. 153. no. 3742, pp. 1381 - 1382
DOI: 10.1126/science.153.3742.1381
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Articles

Electrochromatography with Reversing Electrophoretic Field

Samuel Raymond 1 and John Broome 2

1 Wm. Pepper Laboratory of Clinical Medicine, Hospital of the University of Pennsylvania, Philadelphia
2 Current Incorporated, Philadelphia

A protein mixture can be separated in a flow column by application of a transverse electrophoretic field to carry faster-migrating components of the mixture into a fixed gel bed at one side of the column, so that slower components move along in a buffer stream. The field is then reversed to returnthe faster-moving components to the buffer stream. The cycle is repeated many times to complete the separation.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)