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Science 9 September 1966:
Vol. 153. no. 3741, pp. 1252 - 1254
DOI: 10.1126/science.153.3741.1252
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Articles

Susceptibility of Human Diploid Fibroblast Strains to Transformation by SV40 Virus

George J. Todaro 1, Howard Green 1, and Michael R. Swift 2

1 Department of Pathology, New York University School of Medicine
2 Department of Medicine, New York University School of Medicine, New York 10016

A quantitative system has been developed for the study of transformation of human diploid fibroblasts in culture by two oncogenic viruses, SV40 and the E46 strain of adeno 7-SV40 "hybrid" virus. Seven of the eleven cell strains derived from human skin biopsies when infected with SV40 (109 tissue culture infective doses per milliliter) gave rise to transformed colonies with approximately the same frequency (0.03 percent). Two strains derived from patients with Fanconi's anemia, an autosomal recessive disease associated with a high incidence of chromosome abnormalities and spontaneous neoplasms, gave values more than ten times higher. Two strains from persons heterozygous for this gene were also considerably more susceptible to viral transformation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Human Papillomavirus Type 16 E7 Oncoprotein Activates the Fanconi Anemia (FA) Pathway and Causes Accelerated Chromosomal Instability in FA Cells.
N. Spardy, A. Duensing, D. Charles, N. Haines, T. Nakahara, P. F. Lambert, and S. Duensing (2007)
J. Virol. 81, 13265-13270
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High Incidence of Head and Neck Squamous Cell Carcinoma in Patients With Fanconi Anemia.
D. I. Kutler, A. D. Auerbach, J. Satagopan, P. F. Giampietro, S. D. Batish, A. G. Huvos, A. Goberdhan, J. P. Shah, and B. Singh (2003)
Arch Otolaryngol Head Neck Surg 129, 106-112
   Abstract »    Full Text »    PDF »
Elevated Homologous Recombination Activity in Fanconi Anemia Fibroblasts.
B. Thyagarajan and C. Campbell (1997)
J. Biol. Chem. 272, 23328-23333
   Abstract »    Full Text »    PDF »
Fanconi's Anemia: Tumor-like Warts, Hyperpigmentation Associated With Deranged Keratinocytes, and Depressed Cell-Mediated Immunity.
E. Johansson, K.-M. Niemi, M. Siimes, and S. Pyrhonen (1982)
Arch Dermatol 118, 249-252
   Abstract »    PDF »
Aplastic Anemia: Biology and Treatment.
R. P. GALE, R. E. CHAMPLIN, S. A. FEIG, and J. H. FITCHEN (1981)
Ann Intern Med 95, 477-494
   Abstract »    PDF »
Squamous Cell Carcinomas in Fanconi's Anemia.
J. P. Ortonne, R. Jeune, J. Coiffet, and J. Thivolet (1981)
Arch Dermatol 117, 443-444
   Abstract »    PDF »
Expression of Transforming Viral Genes in Semipermissive Cells Transformed by SV40 or Adenovirus Type 2 or Type 5.
S. J. Flint and G. A. Beltz (1980)
Cold Spring Harb Symp Quant Biol 44, 89-102
   Abstract »    PDF »
Catalytic Properties of the SV40 Large T Antigen.
R. Tjian, A. Robbins, and R. Clark (1980)
Cold Spring Harb Symp Quant Biol 44, 103-111
   Abstract »    PDF »
Enzymatic Activities Associated with the SV40 Large T Antigen.
J. D. Griffin, G. Spangler, and D. M. Livingston (1980)
Cold Spring Harb Symp Quant Biol 44, 113-122
   Abstract »    PDF »
Characterization of a Murine Cellular SV40 T Antigen in SV40-transformed Cells and Uninfected Embryonal Carcinoma Cells.
D. I. H. Linzer, W. Maltzman, and A. J. Levine (1980)
Cold Spring Harb Symp Quant Biol 44, 215-224
   Abstract »    PDF »
SV40-induced Somatic Mutations: Possible Relevance to Viral Transformation.
M. Theile, M. Strauss, L. Luebbe, S. Scherneck, H. Krause, and E. Geissler (1980)
Cold Spring Harb Symp Quant Biol 44, 377-382
   Abstract »    PDF »
X-linked Dyskeratosis Congenita With Pancytopenia.
A. Gutman, A. Frumkin, A. Adam, N. Bloch-Shtacher, and L. A. Rozenszajn (1978)
Arch Dermatol 114, 1667-1671
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SV40 T Antigen: Partial Purification and Properties.
D. M. Livingston, I. C. Henderson, and J. Hudson (1974)
Cold Spring Harb Symp Quant Biol 39, 283-289
   Abstract »    PDF »
Fanconi-Like Syndrome: Immunologic Deficiency, Pancytopenia, and Cutaneous Malignancies.
D. Abels and W. B. Reed (1973)
Arch Dermatol 107, 419-423
   Abstract »    PDF »
Diabetes Mellitus and the Gene for Fanconi's Anemia.
M. Swift, L. Sholman, and D. Gilmour (1972)
Science 178, 308-310
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Squamous Cell Carcinomas in Fanconi's Anemia.
M. Swift, D. Zimmerman, and E. R. McDonough (1971)
JAMA 216, 325-326
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Chromosomal Aberrations in Two Cases of Inherited Aplastic Anemia with Unusual Clinical Features.
R. J. HIRSCHMAN, N. R. SHULMAN, J. G. ABUELO, and J. WHANG-PENG (1969)
Ann Intern Med 71, 107-117
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Viruses in Carcinogenesis.
R. DULBECCO (1969)
Ann Intern Med 70, 1019-1030
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Science. ISSN 0036-8075 (print), 1095-9203 (online)